Enzo Life Sciences的CYTO-ID®綠色/紅色長期細胞示蹤試劑盒采用專有的非共價細胞標記技術，將含有疏水性脂肪鏈的熒光染料穩定地結合入細胞膜的脂質雙分子層中。標記緩沖液對哺乳動物細胞是等滲的，不含去污劑或有機溶劑。染料對細胞沒有毒性，可在細胞中保留長達96小時，并在有絲分裂時傳遞給子細胞。由于該染料不對細胞內的蛋白質進行共價修飾，與基于硫醇反應的氯甲基或胺反應的琥珀酰亞胺酯類熒光染料的分子探針相比，細胞正常的生理反應得到更好的保留。

也可以使用CELLESTIAL®染料進行雙重標記。標記的細胞可以通過共聚焦熒光顯微鏡等儀器進行觀察。此外，染料標記的和未標記的細胞群可通過流式細胞儀進行分析。在延長96小時的培養后，沒有觀察到熒光轉移到相鄰細胞。這與Calcein AM和BCECF AM標記形成鮮明對比，后者在生理溫度下只能在存活的細胞內保留幾個小時。

Enzo Life Sciences的CYTO-ID®長期細胞示蹤試劑盒適用于多種應用，如追蹤細胞譜系，長期細胞活力、細胞毒性、細胞粘附、細胞遷移和細胞-細胞融合研究。

產品特點

● 可使用各種CELLESTIAL® 熒光探針進行雙重標記

● 熒光從染料標記的細胞轉移到未標記的細胞的情況極少

● 適用于長期細胞存活率、細胞毒性、細胞粘附、細胞遷移和細胞間融合測定

實驗示例

圖1.明場（A）和熒光顯微鏡圖像（B）顯示了用CYTO-ID® Green Tracer dye對Jurkat細胞的染色。使用標準的FITC（綠色）濾光片組對膜結合信號進行成像。

圖2.流式細胞術分析Jurkat細胞混合群隨時間變化的熒光。將用 CYTO-ID®綠色示蹤染料染色的Jurkat細胞與未染色的Jurkat細胞群混合并孵育120小時。

圖3.明場（A）和熒光顯微鏡圖像（B）顯示了用CYTO-ID® Red Tracer dye對Jurkat細胞的染色。使用標準的Texas Red（紅色）濾光片組對膜結合信號進行成像。

圖4.熒光顯微鏡圖像顯示了用CYTO-ID® Red Tracer dye對HeLa細胞的染色。使用標準的Texas Red（紅色）濾光片組對膜結合信號進行成像(20x)。

圖5.流式細胞術分析Jurkat細胞混合群隨時間變化的熒光。將用 CYTO-ID®紅色示蹤染料染色的Jurkat細胞與未染色的Jurkat細胞群混合并孵育96小時。

產品信息

部分產品引用文獻

1. Proangiogenic effects of tumor cells on endothelial progenitor cells vary with tumor type in an in vitro and in vivo rat model: R. An, et al.; FASEB J. 32, 5587 (2018), Application(s): Identification of TR-BME2 cells in tube-formation co-culture assay with CC521 colon adenocarcinoma cells

2. A liquid biopsy-based method for the detection and quantification of circulating tumor cells in surgical osteosarcoma patients: H. Zhang , et al.; Int. J. Oncol. 50, 1075 (2017), Application(s): Use with HOS cells (osteosarcoma). Microscopy

3. INBRX-120, a CD8α-targeted detuned IL-2 that selectively expands and activates tumoricidal effector cells for safe and durable in vivo responses: F. J. Sulzmaier, et al.; J. Immunother. Cancer 11, e006116 (2023)

4. Epigenetic modulation of immune synaptic-cytoskeletal networks potentiates γδ T cell-mediated cytotoxicity in lung cancer: R.R. Weng, et al.; Nat. Commun. 12, 2163 (2021)

5. Characterization and Cellular Internalization of Spherical Cellulose Nanocrystals (CNC) into Normal and Cancerous Fibroblasts: N.A.H. Shazali, et al.; Materials (Basel) 12, 3251 (2019), Application(s): C6 and NIH3T3 cell tracing

6. Time resolved 3D live-cell imaging on implants: A. Ingendoh-Tsakmakidis, et al.; PLoS One 13, e0205411 (2018)

7. Nerve guidance conduit with a hybrid structure of a PLGA microfibrousbundle wrapped in a micro/nanostructured membrane: S.W. Peng, et al.; Int. J. Nanomedicine 12, 421 (2017), Application(s):Tracking in vitro cells migration (immortalized neuron progenitor KT 98)

8. Thioreductase-containing epitopes inhibit the development of type 1 diabetes in the NOD mouse model: E. Malek Abrahimians, et al.; Front. Immunol. 7, 67 (2016), Application(s): Flow cytometry analysis using isolated mouse spleen B cells

9. Syngeneic murine ovarian cancer model reveals that ascites enriches for ovarian cancer stem-like cells expressing membrane GRP78: L. Mo, et al.; Mol. Cancer Ther. 14, 747 (2015) 

10. An in vitro blood-brain barrier model combining shear stress and endothelial cell/astrocyte co-culture: Y. Takeshita, et al.; J. Neurosci. Methods 232, 165 (2014), Application(s): Confocal microscopy using adult human astrocyte cells

11. Vascular endothelial growth factor-C modulates proliferation and chemoresistance in acute myeloid leukemic cells through an endothelin-1-dependent induction of cyclooxygenase-2: K.T. Hua, et al.; Biochim. Biophys. Acta 1843, 387 (2014)

12. Expanding the Ig superfamily code for laminar specificity in retina: expression and role of contactins: M. Yamagata, et al.; J. Neurosci. 32, 14402 (2012)

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